Filbertone, (2E)-5-methyl-2-hepten-4-one, regulates thermogenesis and lipid metabolism in skeletal muscle of a high-fat diet fed mice

Abstract Filbertone, the principal flavor compound of hazelnuts, is known to have preventive effects against hypothalamic inflammation, obesity and adipocity in vitro vivo. However, effect filbertone skeletal muscle remains unknown. In present study, we determined mice fed a high-fat diet (HFD). To identify underlying molecular cellular processes filbertone, performed whole transcriptome sequencing muscle. The analysis revealed that upregulated differentially expressed genes (DEGs) filbertone-fed were substantially associated with several pathways including thermogenesis, fatty acid degradation, oxidative phosphorylation, branched chain amino acids (BCAAs) degradation. Furthermore, expression level thermogenic such as uncoupling protein 1 (Ucp1; p < 0.05), cell death-inducing DNA fragmentation factor alpha-like effector A (Cidea; peroxisome proliferator-activated receptor alpha (Ppara; 0.05) lipid droplet-associated Plin3 (p Plin4 Plin5 significantly tissue HFD compared only mice. Filbertone also elevated UCP1 PPARα 0.05). addition, reduced accumulation intracellular lipids C 2 12 myotubes On basis these results, suggest has crucial regulation metabolism energy balance.